12/26/2023 0 Comments Ndm file transfer![]() ![]() We collected the clinical information of the patients who had been cured for the infection. Importantly, we observed 2 clusters of isolates which were the evidences of clonal transmission among patients. Through serial passage for 10 days, we found 5 KPC-2-NDM-1-CRKPs were quite stable to maintain the resistant plasmids. We had the complete genomes sequenced for all the 7 KPC-2-NDM-1-CRKPs, and based on phylogenetic analysis we proposed that KPC-2-NDM-1-CRKP emerged from a KPC-2-CRKP progenitor which acquired another highly transferable bla NDM-1 plasmid later. Our surveillance data allows us to monitor the incidence of KPC-2-NDM-1-CRKP. This study is based on the largest Carbapenem-resistant Enterobacteriaceae (CRE) Network in China, covering the population of 65 hospitals in 25 provinces and municipalities across China for 5 years. Furthermore, how KPC-2-NDM-1-CRKP emerged and clinical treatment practice, were largely unknown. However, no one had provided long term surveillance information, or evidence for stability to maintain KPC-2 and NDM-1, or evidence for cross infection, i.e. All of them were case reports with routine description on the antimicrobial resistance profile and plasmid transferability in vitro, and only one had done genome sequencing. After filtration of literatures without reporting any KPC-2 and NDM-1 coproducing carbapenem-resistant Klebsiella pneumoniae (KPC-2-NDM-1-CRKP), there were only 3 relevant papers describing the emerging of 4 KPC-2-NDM-1-CRKPs in India, Pakistan and China, and an undocumented genome sequence for an extra KPC-2-NDM-1-CRKP. We also blasted bla KPC-2 and bla NDM-1 against NCBI NT and RefSeq Assembly databases for complete genome sequences released up to May 29, 2019. We searched PubMed with the terms “KPC-2 AND NDM-1 AND Klebsiella pneumoniae” and followed the references and citations for reports published up to May 29, 2019, with no language restrictions. ![]()
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